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rabbit anti yap  (Proteintech)


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    Structured Review

    Proteintech rabbit anti yap
    Rabbit Anti Yap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+yap/YAP1+Antibody/pmc12723136-171-35-41
    Average 96 stars, based on 477 article reviews
    rabbit anti yap - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling
    Article Snippet: The rabbit anti YAP (Proteintech, 13584), rabbit anti pYAP S127 (Cell Signaling, 4911), rabbit anti LATS1 (Cell Signaling, 3477), rabbit anti LATS2 (Cell Signaling, 5888), rabbit anti pLATS1-S909 (Cell Signaling, 9157), rabbit anti MST1/2 (Bethyl, A300-466A), and rabbit anti pLATS1T1079 (Cell Signaling, 8654) were used at 1:500.

    Article Title: 2018 ASCB Annual Meeting abstracts
    Article Snippet: The coverslips were stained for YAP (rabbit anti YAP; Proteintech), F actin (Phalloidin; ThermoFisher), and nuclei (DRAQ7; Abcam).

    Incubation:

    Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy
    Article Snippet: An equal amount of 40 μg of protein from each sample was separated by 10 % SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked and then incubated overnight at 4 °C with the following primary antibodies: rabbit anti-Integrin β1 (1:5000; Cat. No. 12594-1-AP, Proteintech, China), rabbit anti-Na+/K + -ATPase (1:1000; Cat. No. AF6109, Affinity, China), rabbit anti-YAP (1:2000; Cat. No. 13584-1-AP, Proteintech, China), and rabbit anti-p-YAP (1:2000; Cat. No. 29018-1-AP, Proteintech, China). .. Rabbit anti-Histone H3 (1:2000; Cat. No. 17168-1-AP, Proteintech, China) and rabbit anti-GAPDH (1:5000; Cat. No. 10494-1-AP, Proteintech, China) were used as internal reference proteins for the nuclear and cytoplasmic fractions, respectively.



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    Piperlongumine (PL) inhibits <t>YAP</t> signaling in triple-negative breast cancer (TNBC) cells. (A) Western blot analysis of YAP protein levels in MDA-MB-231 and BT549 cells treated with PL (0, 1, and 5 μ M) for 24 h. (B) Western blot analysis of YAP and <t>phosphorylated</t> YAP (p-YAP) expression treated with PL in different time points. (C) Western blot analysis of phosphorylated LATS (p-LATS) and phosphorylated AMPK (p-AMPK) at different time points after PL treatment. (D) Representative immunofluorescence images of YAP expression in BT549 and MDA-MB-231 cells treated with PL for 24 h at the indicated concentrations, with quantification of nuclear YAP-positive cells. Scale bar, 50 μ m. * p < 0.05 and ** p < 0.01, as determined by unpaired t -tests.
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    Piperlongumine (PL) inhibits <t>YAP</t> signaling in triple-negative breast cancer (TNBC) cells. (A) Western blot analysis of YAP protein levels in MDA-MB-231 and BT549 cells treated with PL (0, 1, and 5 μ M) for 24 h. (B) Western blot analysis of YAP and <t>phosphorylated</t> YAP (p-YAP) expression treated with PL in different time points. (C) Western blot analysis of phosphorylated LATS (p-LATS) and phosphorylated AMPK (p-AMPK) at different time points after PL treatment. (D) Representative immunofluorescence images of YAP expression in BT549 and MDA-MB-231 cells treated with PL for 24 h at the indicated concentrations, with quantification of nuclear YAP-positive cells. Scale bar, 50 μ m. * p < 0.05 and ** p < 0.01, as determined by unpaired t -tests.
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    Piperlongumine (PL) inhibits <t>YAP</t> signaling in triple-negative breast cancer (TNBC) cells. (A) Western blot analysis of YAP protein levels in MDA-MB-231 and BT549 cells treated with PL (0, 1, and 5 μ M) for 24 h. (B) Western blot analysis of YAP and <t>phosphorylated</t> YAP (p-YAP) expression treated with PL in different time points. (C) Western blot analysis of phosphorylated LATS (p-LATS) and phosphorylated AMPK (p-AMPK) at different time points after PL treatment. (D) Representative immunofluorescence images of YAP expression in BT549 and MDA-MB-231 cells treated with PL for 24 h at the indicated concentrations, with quantification of nuclear YAP-positive cells. Scale bar, 50 μ m. * p < 0.05 and ** p < 0.01, as determined by unpaired t -tests.
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    Piperlongumine (PL) inhibits <t>YAP</t> signaling in triple-negative breast cancer (TNBC) cells. (A) Western blot analysis of YAP protein levels in MDA-MB-231 and BT549 cells treated with PL (0, 1, and 5 μ M) for 24 h. (B) Western blot analysis of YAP and <t>phosphorylated</t> YAP (p-YAP) expression treated with PL in different time points. (C) Western blot analysis of phosphorylated LATS (p-LATS) and phosphorylated AMPK (p-AMPK) at different time points after PL treatment. (D) Representative immunofluorescence images of YAP expression in BT549 and MDA-MB-231 cells treated with PL for 24 h at the indicated concentrations, with quantification of nuclear YAP-positive cells. Scale bar, 50 μ m. * p < 0.05 and ** p < 0.01, as determined by unpaired t -tests.
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    Image Search Results


    Piperlongumine (PL) inhibits YAP signaling in triple-negative breast cancer (TNBC) cells. (A) Western blot analysis of YAP protein levels in MDA-MB-231 and BT549 cells treated with PL (0, 1, and 5 μ M) for 24 h. (B) Western blot analysis of YAP and phosphorylated YAP (p-YAP) expression treated with PL in different time points. (C) Western blot analysis of phosphorylated LATS (p-LATS) and phosphorylated AMPK (p-AMPK) at different time points after PL treatment. (D) Representative immunofluorescence images of YAP expression in BT549 and MDA-MB-231 cells treated with PL for 24 h at the indicated concentrations, with quantification of nuclear YAP-positive cells. Scale bar, 50 μ m. * p < 0.05 and ** p < 0.01, as determined by unpaired t -tests.

    Journal: Translational Oncology

    Article Title: Piperlongumine suppresses YAP-ET-1-CXCL2 signaling to modulates aggressiveness of triple-negative breast cancer cells

    doi: 10.1016/j.tranon.2026.102700

    Figure Lengend Snippet: Piperlongumine (PL) inhibits YAP signaling in triple-negative breast cancer (TNBC) cells. (A) Western blot analysis of YAP protein levels in MDA-MB-231 and BT549 cells treated with PL (0, 1, and 5 μ M) for 24 h. (B) Western blot analysis of YAP and phosphorylated YAP (p-YAP) expression treated with PL in different time points. (C) Western blot analysis of phosphorylated LATS (p-LATS) and phosphorylated AMPK (p-AMPK) at different time points after PL treatment. (D) Representative immunofluorescence images of YAP expression in BT549 and MDA-MB-231 cells treated with PL for 24 h at the indicated concentrations, with quantification of nuclear YAP-positive cells. Scale bar, 50 μ m. * p < 0.05 and ** p < 0.01, as determined by unpaired t -tests.

    Article Snippet: Antibodies for YAP (#12395), phosphorylated (p)-YAP (S127) (#13008), p-YAP (S61) (#75784), p-AMPK (AMPK; #130429), AMPK (#103487), p-LATS1 (S909) (#9157), and LATS1 (#3477) were purchased from Cell Signaling Technology.

    Techniques: Western Blot, Expressing, Immunofluorescence